[1]田建会,丁思华,戚萍,等.卡维地洛抗动脉粥样硬化作用的研究[J].青岛大学学报(医学版),2007,43(01):25-27.[doi:10.11712/qdyxy200701009]
 TIAN JIAN-HUI,DING SI-HUA,QI PING,et al.AN EXPERIMENTAL STUDY OF THE EFFECT OF CARVEDILOL ON ATHEROSCLEROSIS[J].JOURNAL OF QINGDAO UNIVERSITY (MEDICAL SCIENCES),2007,43(01):25-27.[doi:10.11712/qdyxy200701009]
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卡维地洛抗动脉粥样硬化作用的研究()
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《青岛大学学报(医学版)》[ISSN:2096-5532/CN:37-1217/R]

卷:
第43卷
期数:
2007年01期
页码:
25-27
栏目:
出版日期:
2007-02-25

文章信息/Info

Title:
AN EXPERIMENTAL STUDY OF THE EFFECT OF CARVEDILOL ON ATHEROSCLEROSIS
文章编号:
1672-4488(2007)01-0025-03
作者:
田建会1丁思华2戚萍3赵梅2
1 青岛大学医学院附属医院心内科,山东 青岛 266003; 2 青岛大学医学院; 3 青岛大学医学院附属医院动态心电图室
Author(s):
TIAN JIAN-HUI DING SI-HUA QI PING et al
Department of Cardiology, The Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, China
关键词:
动脉硬化卡维地洛抗氧化剂细胞增殖
Keywords:
atherosclerosis carvedilol antioxidants cell proliferation rabbits
分类号:
R972.9
DOI:
10.11712/qdyxy200701009
文献标志码:
A
摘要:
目的 探讨卡维地洛在动脉粥样硬化病变中的抗氧化作用及其对增殖细胞核抗原(PCNA)及核因子kappaBp65(NFκBp65)活化的影响。
方法 雄性新西兰大耳白兔24只,随机分为正常组、高脂组、卡维地洛组,每组8只,共计喂养10周。实验结束后,酶法检测各组家兔的血清三酰甘油及胆固醇,黄嘌呤氧化法检测血清超氧化物歧化酶(SOD),硫代巴比妥酸比色法检测丙二醛(MDA),并采用免疫组化的方法测定血管壁PCNA及NFκBp65的表达。
结果 高脂组和卡维地洛组血清三酰甘油和胆固醇较正常组明显升高(F=146.25、287.83,q=14.76~20.75,P<0.01),而该两组间差别无统计学意义(q=0.53、0.59,P>0.05)。高脂组较正常组SOD明显降低,MDA明显增加,而卡维地洛组较高脂组SOD有所升高,MDA有所降低(F=21.65、75.64,q=4.98~24.29,P<0.01)。高脂组PCNA和NFκBp65的表达较正常组明显增加,卡维地洛组PCNA和NFκBp65的表达较高脂组明显减少(F=426.12、521.38,q=56.15~75.87,P<0.01)。PCNA和NFκBp65的呈正相关(r=0.975,P<0.01)。
结论 卡维地洛有减轻脂质过氧化损伤的作用,可阻断NFκB的活化,进而抑制平滑肌细胞的增殖。
Abstract:
Objective  To study the effect of carvedilol on anti-oxidation and activation of PCAN and NFκB on atherosclerosis.
Methods  Twenty-four male New Zealand white rabbits were randomly divided into three groups: normal group, high-fat diet group and cavedilol-treated group, with eight rabbits in each group. All the rabbits were fed for 10 weeks. After completion of the experiment, the levels of serum triglyceride and cholesterol were measured by enzymatic method, of serum SOD by xanthine oxidative technique, and of serum MDA by thiobarbituric acid method. Expression of the PCAN and NFκBp65 in the vascular wall was detected immunohistochemically.
Results  Compared with those of normal rabbits, the levels of serum triglyceride and cholesterol of the high-fat diet and cavedilol-treated were extremely high (F=146.25,287.83; q=14.76-20.75; P<0.01), but no statistical difference was found between the latter two groups (q=0.53,0.59; P>0.05). Low-level serum SOD and high-level serum MDA were noticed in high-fat diet rabbits. In high-fat diet group, SOD was lower than that in normal group, and MDA was higher; in cavedilol-treated group, SOD was higher, and MDA was lower as compared with normal group (F=21.65,75.64; q=4.98-24.29; P<0.01). The expression of PCNA and NFκBp65 in high-fat diet group was markedly increased as compared with the normal group; but the expression was decreased in carvedilol group as compared with high-fat diet (F=426.12,521.38; q=56.15-75.87; P<0.01). An analysis showed that a positive correlation existed between PCNA and NFκBp65.
Conclusion  Carvedilol might relieve the lipid peroxidation injury and block NFκB activation so as to inhibit the proliferation of smooth muscle cells.
更新日期/Last Update: 2013-12-15