[1]董淑雯,宋海滨,单迅,等.参力保对家兔心肌缺血再灌注损伤的保护作用[J].青岛大学学报(医学版),2008,44(02):99-102.[doi:10.11712/qdyxy200802003]
 DONG SHU-WEN,SONG HAI-BIN,SHAN XUN,et al.PROTECTION OF SHENLIBAO AGAINST MYOCARDIAL ISCHEMIA REPERFUSION INJURY IN RABBITS[J].JOURNAL OF QINGDAO UNIVERSITY (MEDICAL SCIENCES),2008,44(02):99-102.[doi:10.11712/qdyxy200802003]
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参力保对家兔心肌缺血再灌注损伤的保护作用()
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《青岛大学学报(医学版)》[ISSN:2096-5532/CN:37-1217/R]

卷:
第44卷
期数:
2008年02期
页码:
99-102
栏目:
出版日期:
2008-04-25

文章信息/Info

Title:
PROTECTION OF SHENLIBAO AGAINST MYOCARDIAL ISCHEMIA REPERFUSION INJURY IN RABBITS
文章编号:
1672-4488(2008)02-0099-04
作者:
董淑雯宋海滨单迅金毅
青岛大学医学院生理学教研室,山东 青岛 266021
Author(s):
DONG SHU-WEN SONG HAI-BIN SHAN XUN et al 
Department of Physiology, Qingdao University Medical College, Qingdao 266021, China
关键词:
参力保再灌注损伤心肌脂质过氧化损伤
Keywords:
Shenlibao Reperfusion injury Myocardium Lipid peroxidation damage Rabbit
分类号:
R917.7
DOI:
10.11712/qdyxy200802003
文献标志码:
A
摘要:
目的 观察参力保对家兔心肌缺血再灌注损伤的保护作用。
方法 结扎兔冠状动脉左前降支(LAD)30 min,再灌注120 min后制备家兔心肌缺血再灌注模型。56只家兔,随机分为7组,生理盐水对照组:假手术组,模型组(给予溶媒8.58 mg/kg),参力保高剂量组(22.36 mg/kg,内含5.2 mg/kg人参皂苷Rg2),参力保中剂量组(11.18 mg/kg,内含2.6 mg/kg人参皂苷Rg2),参力保低剂量组(5.59 mg/kg,内含1.3 mg/kg人参皂苷Rg2),阳性对照组(给予盐酸尼卡地平注射液),测定血浆磷酸激酸同工酶(CK-MB)、乳酸脱氢酶(LDH)、天门冬氨酸转氨酶(AST)和心肌组织中超氧化物歧化酶(SOD)活性、丙二醛(MDA)及游离脂肪酸 (NFFA) 含量;高效液相法测定心肌组织去甲肾上腺素(NE)含量。
结果 参力保能显著降低血浆CK-MB和LDH、AST的活性,与模型组相比有显著差异(F=13.031~32.604,P<0.05、0.01)。参力保3个剂量组与模型组比较可明显降低心肌MDA含量,提高SOD活性和NE的含量(F=2.880~15.927,q=5.249~12.510,P<0.05);参力保高、中、低剂量组NFFA与模型组比较,差异有显著性(Z1=Z2=Z3=-2.329,P<0.05)。
结论 参力保对家兔心肌缺血再灌注损伤有保护作用,其机制与清除自由基抑制脂质过氧化反应有关。
Abstract:
Objective  To investigate the effect of Shenlibao against myocardial ischemia reperfusion injury in rabbits.
Methods  An animal model was established by ligation of left desending coronary artery for 30 min and reperfusion for 120 min. Fifty-six rabbbits were randomly divided into seven groups: saline control group, sham-operation group, media control group, high-dose Shenlibao group, moderate-dose Shenlibao group, low-dose Shenlibao group and nicardipine hydrochloride treatment group. Serum level of CK-MB and lactate dehydrogenase (LDH), superoxide dismutase (SOD), malondialdehyde (MDA) and free fatty acids (NFFA) were detected.
Results  Compared with the control groups, Shenlibao decreased the activity of CK-MB and LDH in blood plasma (F=13.031-32.604;P<0.05,0.01). Furthermore, Shenlibao of three different-dosage groups increased the activity of superoxide dismutase (SOD) (F=8.765,P<0.01), reduced malondialdehyde (MDA) (F=2.880,P<0.05) and free fatty acids (NFFA) content in myocardium (Z1=Z2=Z3=-2.329,P<0.01), and raised myocardial norepinephrine (NE) contents (F=15.927,P<0.01).
Conclusion  Shenlibao has a protective effect on myocardial ischemia reperfusion injury. The mechanism is related to the clearance of free radicals and inhibition of subsequent lipid peroxidation.
更新日期/Last Update: 2013-12-11