[1]朱蔚文,孙卫文,王卫钢,等.灯盏生脉胶囊对帕金森病大鼠黑质区多巴胺能神经元损伤影响[J].青岛大学医学院学报,2017,53(03):283-286.[doi:10.13361/j.qdyxy.201703009]
 ZHU Weiwen,SUN Weiwen,WANG Weigang,et al.EFFECT OF DENGZHAN SHENGMAI CAPSULES ON DOPAMINERGIC NEURON INJURY IN SUBSTANTIA NIGRA IN RATS WITH PARKINSON’S DISEASE[J].Acta Aacademiae Medicinae Qingdao,2017,53(03):283-286.[doi:10.13361/j.qdyxy.201703009]
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灯盏生脉胶囊对帕金森病大鼠黑质区多巴胺能神经元损伤影响()
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《青岛大学医学院学报》[ISSN:1672-4488/CN:37-1356/R]

卷:
第53卷
期数:
2017年03期
页码:
283-286
栏目:
出版日期:
2017-08-07

文章信息/Info

Title:
EFFECT OF DENGZHAN SHENGMAI CAPSULES ON DOPAMINERGIC NEURON INJURY IN SUBSTANTIA NIGRA IN RATS WITH PARKINSON’S DISEASE
文章编号:
1672-4488(2017)03-0283-04
作者:
朱蔚文孙卫文王卫钢梁采宇李嘉滢
广州医科大学附属第二医院神经内科,广州 510260
Author(s):
ZHU Weiwen SUN Weiwen WANG Weigang LIANG Caiyu LI Jiaying
Department of Neurology, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510260, China
关键词:
灯盏生脉胶囊帕金森病黑质氧化嘌呤核苷磷酸酶酪氨酸羟化酶
Keywords:
Dengzhan Shengmai capsule Parkinson’s disease substantia nigra oxidized purine nucleoside triphosphatase tyrosine hydroxylase
分类号:
R742.5
DOI:
10.13361/j.qdyxy.201703009
文献标志码:
A
摘要:
目的 研究灯盏生脉胶囊对6-羟基多巴胺(6-OHDA)诱导的帕金森病(PD)大鼠黑质多巴胺能神经元损伤的影响,并探讨其与DNA氧化损伤修复的关系。
方法 将80只大鼠随机分为假手术组、模型组、灯盏生脉低剂量组(低剂量组)、灯盏生脉高剂量组(高剂量组)。采用6-OHDA纹状体注射法制备PD大鼠模型。灯盏生脉组在造模前3 d开始灌胃给予低剂量、高剂量灯盏生脉至第5周处死大鼠前,假手术组、模型组同法给予等剂量的生理盐水。造模后第4周进行阿扑吗啡诱发转圈实验。第5周大鼠断头取脑后,采用Western blot方法和免疫组织化学方法,检测黑质区酪氨酸羟化酶(TH)和氧化嘌呤核苷磷酸酶(MTH1)的阳性细胞数量及其蛋白表达量。
结果 4组间转圈率比较,差异有显著性(χ2=21.89,P<0.05)。假手术组手术侧黑质致密部TH细胞数为78±13,模型组为27±7,低剂量组为39±8,高剂量组为54±11,4组间TH阳性细胞数比较差异有显著性(F=43.89,P<0.05)。高剂量组手术侧黑质区TH蛋白含量明显高于模型组(F=9.50,P<0.05)。假手术组手术侧黑质致密部MTH1阳性细胞数为42±7,模型组为22±6,低剂量组为36±6,高剂量组为45±8,4组间MTH1阳性细胞数比较差异有显著性(F=22.65,P<0.05)。高剂量组手术侧黑质区MTH1蛋白含量最多,假手术组最少,两组比较差异有显著性(F=50.49,P<0.05)。
结论 灯盏生脉胶囊能有效减轻PD大鼠黑质多巴胺能神经元损伤,其作用机制与提高DNA损伤修复有关。
Abstract:
Objective  To investigate the effect of Dengzhan Shengmai capsules on dopaminergic neuron injury in the substantia nigra in rats with Parkinson’s disease (PD) induced by 6-hydroxydopamine (6-OHDA), as well as its mechanism in DNA oxidative damage repair.
Methods  A total of 80 rats were randomly divided into sham-operated group, model group, low-dose Dengzhan Shengmai group (low-dose group), and high-dose Dengzhan Shengmai group (high-dose group). Injection of 6-OHDA into the striatum was performed to establish a rat model of PD. The rats in the low- and high-dose Dengzhan Shengmai groups were given low- or high-dose Dengzhan Shengmai capsules by gavage from 3 days before modeling to week 5 before sacrifice, and those in the sham-operation group and the model group were given normal saline at the same dose. The changes in rotational behaviors induced by apomorphine were observed at week 4 after modeling. After the rats were beheaded and brain tissue was collected at week 5, immunohistochemistry and Western blot were used to measure the number of cells with tyrosine hydroxylase (TH) or MTH1 in the substantia nigra and their protein expression levels.
Results  There was a significant difference in the rotation rate between the four groups (χ2=21.89,P<0.05). The number of cells with TH in substantia nigra pars compacta at the operation side was 78±13 in the sham-operation group, 27±7 in the model group, 39±8 in the low-dose group, and 54±11 in the high-dose group; there was a significant difference between the four groups (F=43.89,P<0.05). The high-dose group had a significantly higher content of TH protein in the substantia nigra at the operation side than the model group (F=9.50,P<0.05). The number of cells with MTH1 in the substantia nigra pars compacta at the operation side was 42±7 in the sham-operation group, 22±6 in the model group, 36±6 in the low-dose group, and 45±8 in the high-dose group; there was a significant difference between the four groups (F=22.65,P<0.05). The high-dose group had the highest MTH1 content in the substantia nigra at the operation side, while the sham-operation group had the lowest content, and there was a significant difference between these two groups (F=50.49,P<0.05).
Conclusion  Dengzhan Shengmai capsules can effectively alleviate dopaminergic neuron injury in the substantia nigra in rats with PD, and its mechanism is associated with the improvement in DNA damage repair.
更新日期/Last Update: 2017-08-13