[1]朱莹莹,王麟,凌勇,等.促育生精方治疗小鼠不育症的效果及其机制[J].青岛大学医学院学报,2017,53(03):300-304.[doi:10.13361/j.qdyxy.201703014]
 ZHU Yingying,WANG Lin,LING Yong,et al.EFFECT OF CUYU SHENGJING PRESCRIPTION IN TREATMENT OF INFERTILITY IN MICE AND ITS MECHANISM[J].Acta Aacademiae Medicinae Qingdao,2017,53(03):300-304.[doi:10.13361/j.qdyxy.201703014]
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促育生精方治疗小鼠不育症的效果及其机制()
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《青岛大学医学院学报》[ISSN:1672-4488/CN:37-1356/R]

卷:
第53卷
期数:
2017年03期
页码:
300-304
栏目:
出版日期:
2017-08-07

文章信息/Info

Title:
EFFECT OF CUYU SHENGJING PRESCRIPTION IN TREATMENT OF INFERTILITY IN MICE AND ITS MECHANISM
文章编号:
1672-4488(2017)03-0300-05
作者:
朱莹莹1王麟2凌勇2孙立波2孙向红2
青岛大学,山东 青岛 266500; 1 药学院药剂学系; 2 附属医院药剂科
Author(s):
ZHU Yingying WANG Lin LING Yong SUN Libo SUN Xianghong
Department of Pharmaceutical, College of Pharmacy, Qingdao University, Qingdao 266500, China
关键词:
促育生精方cAMP应答元件调节器cAMP反应元件结合蛋白环磷酰胺不育
Keywords:
cuyushengjingfang cyclic amp response element modulator cyclic amp response element-binding protein cyclophosphamide infertility
分类号:
R965
DOI:
10.13361/j.qdyxy.201703014
文献标志码:
A
摘要:
目的 研究促育生精方治疗小鼠不育症的效果及其机制。
方法 雄性清洁级昆明小鼠50只,随机分为空白对照组、模型组和促育生精方干预低剂量组、中剂量组、高剂量组,采用环磷酰胺(CP)造模方法建立小鼠少弱精症模型,促育生精方干预各剂量组在造模过程中给予促育生精方干预,共治疗35 d。光学显微镜下观察各组精子密度与精子活力,采用RT-PCR法检测各组小鼠睾丸组织中
cAMP反应元件调节因子(CREM)mRNA、cAMP反应元件结合蛋白(CREB)mRNA、睾丸特异性CREM激活因子(ACT)mRNA的表达;用Western blotting方法检测各组ACT、CREM、CREB蛋白的表达。
结果 模型组精子密度与精子活力较空白对照组显著降低(F=2.83~121.16,P<0.05),小鼠少弱精症模型制备成功。促育生精方干预中剂量组、高剂量组精子密度显著高于模型组(F=121.16,P<0.05),低剂量组与模型组比较差异无显著性(P>0.05)。促育生精方干预低剂量组、中剂量组精子活力显著高于模型组(F=2.83、18.94,P<0.05),而高剂量组与模型组比较差异无显著性(P>0.05)。模型组ACT、CREM、CREB mRNA及蛋白表达量较空白对照组均显著降低(F=16.28~154.32,P<0.05)。与模型组比较,促育生精方干预中剂量组ACT mRNA和蛋白表达显著升高(F=29.04、64.28,P<0.05),而低剂量、高剂量组差异无显著性(P>0.05);低、中剂量组CREM mRNA和蛋白表达显著升高(F=17.77、41.83,P<0.05),高剂量组差异无显著意义(P>0.05);低剂量组CREB mRNA及蛋白表达显著升高(F=16.89、154.32,P<0.05),而中、高剂量组比较差异无显著意义(P>0.05)。
结论 促育生精方能有效提高少弱精症模型小鼠睾丸组织中ACT、CREM、CREB mRNA及蛋白的表达,改善小鼠精子质量。
Abstract:
Objective  To investigate the effect of Cuyu Shengjing Prescription in the treatment of infertility in mice and its mechanism.
Methods  A total of 50 clean male Kunming mice were randomly divided into blank control group, model group, and low-, medium-, and high-dose Cuyu Shengjing Prescription groups. Cyclophosphamide was used to establish a mouse model of oligoasthenospermia, and the Cuyu Shengjing Prescription groups were given Cuyu Shengjing Prescription for 35 d. An optical microscope was used to observe sperm density and motility, RT-PCR was used to measure the mRNA expression of activator of cAMP-responsive element modulator in testis (ACT), cAMP-responsive element modulator (CREM), and cAMP response element-binding protein (CREB) in testicular tissue, and Western blot was used to measure the protein expression of ACT, CREM, and CREB.
Results  The model group had significantly lower sperm density and motility than the normal group (F=2.83-121.16,P<0.05), suggesting that the mouse model of oligoasthenospermia was established successfully. The medium- and high-dose Cuyu Shengjing Prescription groups had significantly higher sperm density than the model group (F=121.16,P<0.05), while there was no significant difference between the low-dose Cuyu Shengjing Prescription group and the model group (P>0.05). The low- and medium-dose Cuyu Shengjing Prescription groups had significantly higher sperm motility than the model group (F=2.83,18.94;P<0.05), while there was no significant difference between the high-dose Cuyu Shengjing Prescription group and the model group (P>0.05). The model group had significantly lower mRNA and protein expression of ACT, CREM, and CREB than the blank control group (F=16.28-154.32,P<0.05). The medium-dose Cuyu Shengjing Prescription group had significant increases in the mRNA and protein expression of ACT compared with the model group (F=29.04,64.28;P<0.05), while there were no significant differences between the low- and high-dose Cuyu Shengjing Prescription groups and the model group (P>0.05). Compared with the model group, the low- and medium-dose Cuyu Shengjing Prescription groups had significant increases in the mRNA and protein expression of CREM (F=17.77,41.83;P<0.05), while there were no significant differences between the high-dose Cuyu Shengjing Prescription group and the model group (P>0.05). Compared with the model group, the low-dose Cuyu Shengjing Prescription group had significant increases in the mRNA and protein expression of CREB (F=16.89,154.32;P<0.05), while there were no significant differences between the medium- and high-dose Cuyu Shengjing Prescription groups and the model group (P>0.05).
Conclusion  Cuyu Shengjing Prescription can effectively improve the mRNA and protein expression of ACT, CREM, and CREB in testicular tissue in mice with oligoasthenospermia and improve sperm quality.
更新日期/Last Update: 2017-08-13