[1]谢敏,谷万里.大蒜素对apoE-/-小鼠动脉粥样硬化易损斑块细胞凋亡及P53蛋白表达的影响[J].青岛大学医学院学报,2017,53(03):261-263,268.[doi:10.13361/j.qdyxy.201703003]
 XIE Min,GU Wanli.EFFECT OF GARLICIN ON CELL APOPTOSIS AND P53 EXPRESSION IN VULNERABLE PLAQUES IN APOLIPOPROTEIN E GENE KNOCKOUT MICE WITH ATHEROSCLEROSIS[J].Acta Aacademiae Medicinae Qingdao,2017,53(03):261-263,268.[doi:10.13361/j.qdyxy.201703003]
点击复制

大蒜素对apoE-/-小鼠动脉粥样硬化易损斑块细胞凋亡及P53蛋白表达的影响()
分享到:

《青岛大学医学院学报》[ISSN:1672-4488/CN:37-1356/R]

卷:
第53卷
期数:
2017年03期
页码:
261-263,268
栏目:
出版日期:
2017-08-07

文章信息/Info

Title:
EFFECT OF GARLICIN ON CELL APOPTOSIS AND P53 EXPRESSION IN VULNERABLE PLAQUES IN APOLIPOPROTEIN E GENE KNOCKOUT MICE WITH ATHEROSCLEROSIS
文章编号:
1672-4488(2017)03-0261-04
作者:
谢敏1谷万里2
1 青岛大学医学院中西医结合专业,山东 青岛 266003; 2 聊城市人民医院中医科
Author(s):
XIE Min GU Wanli
Integrated Traditional Chinese and Western Medicine, Qingdao University Medical College, Qingdao 266003, China
关键词:
斑块动脉粥样硬化大蒜素载脂蛋白E类小鼠基因敲除基因p53
Keywords:
plaque atherosclerotic garlicin apolipoproteins E mice knockout genes P53
分类号:
R256.2;R282.71
DOI:
10.13361/j.qdyxy.201703003
文献标志码:
A
摘要:
目的 探讨大蒜素对载脂蛋白E基因敲除(apoE-/-)小鼠动脉粥样硬化易损斑块的作用及其机制。
方法 以高脂饲料喂养apoE-/-小鼠8周造模,并随机分为模型组、低剂量大蒜素组和高剂量大蒜素组,每组8只。模型组小鼠给予0.50 mL生理盐水灌胃,低剂量大蒜素组给予0.25 mL生理盐水+0.25 mL大蒜素溶液灌胃,高剂量大蒜素组给予0.50 mL大蒜素溶液灌胃,每天1次,连续8周。然后取主动脉根部,采用TUNEL染色检测斑块凋亡阳性面积,采用免疫组化方法检测斑块内凋亡巨噬细胞、血管平滑肌细胞含量及凋亡相关蛋白P53的表达。
结果 大蒜素灌胃处理后斑块凋亡阳性面积均减小,其中低剂量大蒜素组减小具有统计学意义(F=42.32,P<0.05),高剂量大蒜素组减小差异无统计学意义(P>0.05)。低剂量大蒜素组斑块内凋亡巨噬细胞含量较模型组和高剂量大蒜素组降低,差异具有统计学意义(F=28.13,P<0.05);各组斑块内凋亡血管平滑肌细胞含量比较差异无统计学意义(P>0.05)。低剂量大蒜素组和高剂量大蒜素组斑块内P53蛋白表达均显著低于模型组,差异有统计学意义(F=22.11,P<0.05)。
结论 大蒜素可通过下调P53蛋白的表达、抑制细胞凋亡,发挥稳定晚期不稳定斑块的作用。
Abstract:
Objective  To investigate the effect of garlicin on vulnerable plaques in apolipoprotein E gene knockout (apoE-/-) mice with atherosclerosis and possible mechanisms.
Methods  The apoE-/- mice were given high-fat feed for 8 weeks to establish a mouse model of atherosclerosis, and then these mice were randomly divided into model group, low-dose garlicin group, and high-dose garlicin group, with 8 mice in each group. The mice in the model group were given 0.50 mL normal saline by gavage, those in the low-dose garlicin group were given 0.25 mL normal saline and 0.25 mL garlicin solution by gavage, and those in the high-dose garlicin group were given 0.50 mL garlicin solution by gavage once a day for 8 consecutive weeks. TUNEL staining was used to observe the area of plaque apoptosis in the aortic root, and immunohistochemistry was used to observe the numbers of apoptotic macrophages and vascular smooth muscle cells and the expression of apoptosis-related protein P53.
Results Both low- and high-dose garlicin groups had a reduction in the area of plaque apoptosis after garlicin treatment by gavage; the low-dose garlicin group had a significant reduction (F=42.32,P<0.05), while the high-dose garlicin group had no significant reduction (P>0.05). Compared with the model group and the high-dose garlicin group, the low-dose garlicin group had a significant reduction in the number of apoptotic macrophages in the plaque (F=28.13,P<0.05); there was no significant difference in the number of apoptotic vascular smooth muscle cells in the plaque between groups (P>0.05). The low- and high-dose garlicin groups had significantly lower expression of P53 in the plaque than the model group (F=22.11,P<0.05).
Conclusion  Garlicin can stabilize the advanced unstable plaques by downregulating P53 expression and inhibiting cell apoptosis.
更新日期/Last Update: 2017-08-13