[1]赵凯,董大海,骆磊,等.靶向抑制miR-106b对体外膀胱癌细胞增殖和凋亡影响[J].青岛大学医学院学报,2017,53(03):297-299,304.[doi:10.13361/j.qdyxy.201703013]
 ZHAO Kai,DONG Dahai,LUO Lei,et al.EFFECT OF TARGETED INHIBITION OF MICRORNA-106B ON PROLIFERATION AND APOPTOSIS OF IN VITRO BLADDER CANCER CELLS[J].Acta Aacademiae Medicinae Qingdao,2017,53(03):297-299,304.[doi:10.13361/j.qdyxy.201703013]
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靶向抑制miR-106b对体外膀胱癌细胞增殖和凋亡影响()
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《青岛大学医学院学报》[ISSN:1672-4488/CN:37-1356/R]

卷:
第53卷
期数:
2017年03期
页码:
297-299,304
栏目:
出版日期:
2017-08-07

文章信息/Info

Title:
EFFECT OF TARGETED INHIBITION OF MICRORNA-106B ON PROLIFERATION AND APOPTOSIS OF IN VITRO BLADDER CANCER CELLS
文章编号:
1672-4488(2017)03-0297-04
作者:
赵凯董大海骆磊王永华杨晓坤
青岛大学附属医院泌尿外科,山东 青岛 266003
Author(s):
ZHAO Kai DONG Dahai LUO Lei WANG Yonghua YANG Xiaokun
Department of Urology, The Affiliated Hospital of Qingdao University, Qingdao 266003, China
关键词:
膀胱肿瘤miR-106b细胞增殖细胞凋亡
Keywords:
urinary bladder neoplasms miR-106b proliferation apoptosis
分类号:
R737.14
DOI:
10.13361/j.qdyxy.201703013
文献标志码:
A
摘要:
目的 研究靶向抑制微小RNA-106b(miR-106b)对体外膀胱癌细胞增殖、侵袭和凋亡的影响。
方法 通过细胞转染技术构建靶向抑制miR-106b的膀胱癌细胞克隆,利用实时荧光定量PCR(qRT-PCR)技术检测miR-106b的沉默效果。将细胞分为空白对照组(A组)、阴性对照组(B组)和miR-106b小分子抑制剂转染组(C组)3组,采用细胞增殖实验和克隆形成实验检测靶向抑制miR-106b对膀胱癌T24细胞生长的影响;采用Annexin V/PI检测靶向抑制miR-106b对细胞凋亡的影响;采用细胞迁移实验和侵袭实验检测靶向抑制miR-106b对体外T24细胞侵袭的影响。
结果 C组T24细胞内miR-106b表达水平、细胞存活率、细胞克隆形成数、细胞凋亡率及跨膜细胞数与A、B组比较,差异有显著性(F=37.24~87.89,q=9.57~16.53,P<0.05)。
结论 通过靶向抑制miR-106b可抑制体外膀胱癌细胞增殖、侵袭和凋亡,miR-106b是膀胱癌治疗的基因靶点。
Abstract:
Objective  To investigate the effect of targeted inhibition of microRNA-106b (miR-106b) on the proliferation, invasion, and apoptosis of in vitro bladder cancer cells.
Methods  Cell transfection was used to construct the colonies of bladder cancer cells with targeted inhibition of miR-106b, and quantitative real-time PCR was used to detect the silence of miR-106b. The bladder cancer cells were divided into blank control group (group A), negative control group (group B), and miR-106b small-molecule inhibitor transfection group (group C). Cell proliferation assay and colony-forming assay were used to measure the effect of targeted inhibition of miR-106b on the growth of bladder cancer T24 cell, Annexin V/PI was used to measure the effect on the apoptosis of T24 cells, and cell migration assay and invasion assay were used to measure the effect on the invasion of in vitro T24 cells.
Results  There were significant differences in miR-106b expression, survival rate of cells, number of cell colonies formed, cell apoptosis rate, and number of cells across membrane between group C and groups A/B (F=37.24-87.89,q=9.57-16.53,P<0.05).
Conclusion  Targeted inhibition of miR-106b can inhibit the proliferation, invasion, and apoptosis of bladder cancer cells in vitro, and miR-106b is the target gene for the treatment of bladder cancer.
更新日期/Last Update: 2017-08-13