[1]张玥,曲艳,马浩越,等.中枢Nesfatin-1对脂肪组织UCP-1表达影响及机制[J].青岛大学学报(医学版),2017,53(06):631-634,639.[doi:10.13361/j.qdyxy.201706001]
 ZHANG Yue,QU Yan,MA Haoyue,et al.EFFECTS OF CENTRAL NESFATIN-1 ON UCP-1 EXPRESSION IN BROWN ADIPOSE TISSUES AND WHITE ADIPOSE TISSUES AND UNDERLING MECHANISMS[J].JOURNAL OF QINGDAO UNIVERSITY (MEDICAL SCIENCES),2017,53(06):631-634,639.[doi:10.13361/j.qdyxy.201706001]
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中枢Nesfatin-1对脂肪组织UCP-1表达影响及机制()
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《青岛大学学报(医学版)》[ISSN:2096-5532/CN:37-1217/R]

卷:
第53卷
期数:
2017年06期
页码:
631-634,639
栏目:
出版日期:
2018-03-21

文章信息/Info

Title:
EFFECTS OF CENTRAL NESFATIN-1 ON UCP-1 EXPRESSION IN BROWN ADIPOSE TISSUES AND WHITE ADIPOSE TISSUES AND UNDERLING MECHANISMS
文章编号:
1672-4488(2017)06-0631-05
作者:
张玥1曲艳2马浩越1徐岩3董静1
青岛大学基础医学院,山东 青岛 266071 1 特种医学系; 2 生理学与病理生理学系; 3 附属医院肾内科
Author(s):
ZHANG Yue QU Yan MA Haoyue XU Yan DONG Jing
School of Basic Medicine, Qingdao University, Qingdao 266071, China
关键词:
Nucleobindin脂肪组织解偶联蛋白1下丘脑肾上腺素能纤维
Keywords:
Nucleobindin adipose tissue uncoupling protein 1 hypothalamus sympathetic fibers postganglionic
分类号:
R335.9
DOI:
10.13361/j.qdyxy.201706001
文献标志码:
A
摘要:
目的 了解中枢Nesfatin-1能否通过交感神经促进外周褐色脂肪组织及白色脂肪组织中解偶联蛋白-1(UCP-1)表达,并探讨其中枢分子机制。
方法 将昆明小鼠随机分为生理盐水组(n=6)、Nesfatin-1组(n=7)、生理盐水+阻断剂组(n=6)和Nesfatin-1+阻断剂组(n=7)。4组小鼠均给予下丘脑腹内侧核(VMH)埋管,恢复3 d后,分别腹腔注射生理盐水、生理盐水、β3受体阻断剂、β3受体阻断剂0.2 mL,并于30 min后VMH微量注射生理盐水、Nesfatin-1(1.5×10-8 mol/L)、生理盐水、Nesfatin-1(1.5×10-8 mol/L)0.2 μL,连续3 d。应用Western Blot和RT-PCR方法,检测各组小鼠附睾白色脂肪组织及肩胛部褐色脂肪组织中UCP-1蛋白和mRNA表达水平,以及下丘脑磷酸化腺苷酸活化蛋白激酶(AMPK)的表达。
结果 Nesfatin-1组脂肪组织中UCP-1蛋白、mRNA表达水平及下丘脑磷酸化AMPK蛋白水平较生理盐水组明显升高,Nesfatin-1+阻断剂组较Nesfatin-1组明显降低,差异有显著性(F=3.461~8.808,P<0.01)。
结论 VMH核团微量注射Nesfatin-1可以通过下丘脑-交感神经通路促进外周褐色脂肪组织及白色脂肪组织中UCP-1表达。
Abstract:
Objective  To investigate whether central Nesfatin-1 can promote the expression of uncoupling protein-1 (UCP-1) in peripheral brown adipose tissues (BAT) and white adipose tissues (WAT) through the sympathetic nerve pathway, and to explore the underling mechanisms.
Methods  A total of 26 Kunming mice were randomly divided into the following four groups: normal saline group (n=6), Nesfatin-1 group (n=7), normal saline + β3 receptor antagonist group (n=6), and Nesfatin-1 + β3 receptor antagonist group (n=7). The mice in each group were treated by burying a pipe in the ventromedial hypothalamic nucleus (VMH), and then were intraperitoneally injected with normal saline 0.2 mL, normal saline 0.2 mL, β3 receptor antagonist (2 mg?kg-1?d-1) 0.2 mL, and β3 receptor antagonist (2 mg?kg-1?d-1) 0.2 mL, respectively, at three days after the resumption; after 30 minutes, the mice in each group were microinjected with normal saline 0.2 μL, Nesfatin-1 (1.5×10-8 mol/L) 0.2 μL, normal saline 0.2 μL, and Nesfatin-1 (1.5×10-8 mol/L) 0.2 μL in the VMH, respectively, for three consecutive days. Western blot and RT-PCR were used to measure the protein and mRNA expression of UCP-1 in the epididymal WAT and scapular BAT and WAT as well as the expression of phosphorylated AMP-activated protein kinase (AMPK) in the hypothalamus in each group.
Results  The Nesfatin-1 group had significantly higher protein and mRNA expression of UCP-1 in the epididymal WAT and protein expression of phosphorylated AMPK in the hypothalamus compared with the normal saline group. The Nesfatin-1 + β3 receptor antagonist group had significantly lower protein and mRNA expression of UCP-1 in the epididymal WAT and protein expression of phosphorylated AMPK in the hypothalamus compared with the Nesfatin-1 group (F=3.461-8.808,P<0.01).
Conclusion  Microinjection of Nesfatin-1 into the VMH nucleus can promote the expression of UCP-1 in peripheral BAT and WAT through the hypothalamic-sympathetic nerve pathway.
更新日期/Last Update: 2018-03-24