[1]滕金龙,王立,苏媛,等.G-CSF并EPO对MODS小鼠肝细胞凋亡影响[J].青岛大学学报(医学版),2017,53(06):652-655.[doi:10.13361/j.qdyxy.201706006]
 TENG Jinlong,WANG Li,SU Yuan,et al.EFFECT OF GRANULOCYTE COLONY-STIMULATING FACTOR COMBINED WITH ERYTHROPOIETIN ON LIVER CELL APOPTOSIS IN MICE WITH MULTIPLE ORGAN DYSFUNCTION SYNDROME[J].JOURNAL OF QINGDAO UNIVERSITY (MEDICAL SCIENCES),2017,53(06):652-655.[doi:10.13361/j.qdyxy.201706006]
点击复制

G-CSF并EPO对MODS小鼠肝细胞凋亡影响()
分享到:

《青岛大学学报(医学版)》[ISSN:2096-5532/CN:37-1217/R]

卷:
第53卷
期数:
2017年06期
页码:
652-655
栏目:
出版日期:
2018-03-21

文章信息/Info

Title:
EFFECT OF GRANULOCYTE COLONY-STIMULATING FACTOR COMBINED WITH ERYTHROPOIETIN ON LIVER CELL APOPTOSIS IN MICE WITH MULTIPLE ORGAN DYSFUNCTION SYNDROME
文章编号:
1672-4488(2017)06-0652-04
作者:
滕金龙1王立1苏媛1孙桂霞1高倩2于海初3
1 青岛大学附属医院重症医学科,山东 青岛 266003; 2 青岛市第三人民医院重症医学科; 3 青岛大学附属医院心血管内科
Author(s):
TENG Jinlong WANG Li SU Yuan SUN Guixia GAO Qian YU Haichu
Department of Intensive Care Unit, The Affiliated Hospital of Qingdao University, Qingdao 266003, China
关键词:
多器官功能衰竭阿法依泊汀粒细胞集落刺激因子肝细胞细胞凋亡
Keywords:
multiple organ failure epoetin alfa granulocyte colony-stimulating factor hepatocytes apoptosis
分类号:
R365;R329.25
DOI:
10.13361/j.qdyxy.201706006
文献标志码:
A
摘要:
目的 探讨粒细胞集落刺激因子(G-CSF)并促红细胞生成素(EPO)对多器官功能障碍综合征(MODS)小鼠肝细胞凋亡的影响。
方法 取健康雄性C57BL/6小鼠125只,随机分为正常对照组(N组)、MODS组(M组)、MODS+EPO组(M+E组)、MODS+G-CSF组(M+G组)和MODS+EPO+G-CSF组(M+E+G组)5组。采用腹腔注射酵母多糖的方法制作MODS模型。建模后第11天提取小鼠的肝组织,采用Western-blot方法检测各组肝组织中Bax、Bcl-2、Caspase-3蛋白的表达水平;采用原位末端标记技术,观察细胞凋亡情况,并计算凋亡指数(AI)。
结果 M+E组、M+G组、M+E+G组凋亡蛋白Bax、Caspase-3的表达明显低于M组,差异有显著性(F=120.66,q=2.96~12.83,P<0.05);M+E+G组Bax、Caspase-3蛋白的表达明显低于M+E组、M+G组,差异有显著性(q=7.39~8.87,P<0.05)。M+E组、M+G组、M+E+G组抗凋亡蛋白Bcl-2的表达明显高于M组,差异有显著性(F=113.32,q=5.38~19.92,P<0.05);M+E+G组Bcl-2蛋白的表达明显高于M+E组、M+G组,差异有显著性(q=12.65~14.55,P<0.05)。M+E组、M+G组、M+E+G组的AI明显低于M组,差异有显著性(F=73.31,q=9.84~20.86,P<0.05)。
结论 G-CSF和EPO均能在一定程度上改善MODS小鼠的肝细胞凋亡,二者联用效果优于单独使用其中任一种药物。其抗凋亡机制可能是通过上调抗凋亡蛋白Bcl-2,以及下调凋亡蛋白Bax和Caspase-3的表达来实现的。
Abstract:
Objective  To investigate the effect of granulocyte colony-stimulating factor (G-CSF) combined with erythropoietin (EPO) on liver cell apoptosis in mice with multiple organ dysfunction syndrome (MODS).
Methods  A total of 125 healthy male C57BL/6 mouse were randomly divided into normal control group (N group), MODS group (M group), MODS+EPO group (M+E group), MODS+G-CSF group (M+G group), and MODS+EPO+G-CSF group (M+E+G group). Intraperitoneal injection of yeast polysaccharides was performed to establish a model of MODS. Liver tissue was collected on day 11 after the model was established. Western blot was used to measure the protein expression of Bax, Bcl-2, and Caspase-3 in liver tissue, and in situ end-labeling was used to observe cell apoptosis and calculate apoptotic index (AI).
Results  The M+E group, the M+G group, and the M+E+G group had significantly lower protein expression of Bax and Caspase-3 than the M group (F=120.66,q=2.96-12.83,P<0.05); the M+E+G group had significantly lower protein expression of Bax and Caspase-3 than the M+E group and the M+G group (q=7.39-8.87,P<0.05). The M+E group, the M+G group, and the M+E+G group had significantly higher expression of Bcl-2 than the M group (F=113.32,q=5.38-19.92,P<0.05); the M+E+G group had significantly higher expression of Bcl-2 than the M+E group and the M+G group (q=12.65-14.55,P<0.05). The M+E group, the M+G group, and the M+E+G group had a significantly lower AI than the M group (F=73.31,q=9.84-20.86,P<0.05).
Conclusion  Both G-CSF and EPO can improve liver cell apoptosis in MODS mice, and a combination of G-CSF and EPO has a better effect than G-CSF or EPO alone. The anti-apoptosis mechanism of G-CSF and EPO may be realized by upregulating the expression of Bcl-2 and downregulating the expression of Bax and Caspase-3.
更新日期/Last Update: 2018-03-24