[1]唐彬,宋振梅,肖琦凡.黄芩苷对高脂-脂肪肝模型大鼠肝脏炎症改善及炎性因子表达的影响[J].青岛大学学报(医学版),2017,53(06):659-663,667.[doi:10.13361/j.qdyxy.201706008]
 TANG Bin,SONG Zhenmei,XIAO Qifan.EFFECT OF BAICALIN ON THE IMPROVEMENT OF LIVER INFLAMMATION AND THE EXPRESSION OF INFLAMMATORY FACTORS IN A RAT MODEL OF HYPERLIPIDEMIA-FATTY LIVER DISEASE[J].JOURNAL OF QINGDAO UNIVERSITY (MEDICAL SCIENCES),2017,53(06):659-663,667.[doi:10.13361/j.qdyxy.201706008]
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黄芩苷对高脂-脂肪肝模型大鼠肝脏炎症改善及炎性因子表达的影响()
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《青岛大学学报(医学版)》[ISSN:2096-5532/CN:37-1217/R]

卷:
第53卷
期数:
2017年06期
页码:
659-663,667
栏目:
出版日期:
2018-03-21

文章信息/Info

Title:
EFFECT OF BAICALIN ON THE IMPROVEMENT OF LIVER INFLAMMATION AND THE EXPRESSION OF INFLAMMATORY FACTORS IN A RAT MODEL OF HYPERLIPIDEMIA-FATTY LIVER DISEASE
文章编号:
1672-4488(2017)06-0659-06
作者:
唐彬1宋振梅2肖琦凡1
中日友好医院,北京 100029 1 国际部; 2 消化科
Author(s):
TANG Bin SONG Zhenmei XIAO Qifan
International Department of China-Japan Friendship Hospital, Beijing 100029, China
关键词:
黄芩甙脂肪肝膳食高脂肿瘤坏死因子α白细胞介素6环氧化酶2大鼠Wistar
Keywords:
baicalin fatty liver diet high-fat tumor necrosis factor-alpha interleukin-6 cyclooxygenase 2 rats Wistar
分类号:
R285.5;R575.5
DOI:
10.13361/j.qdyxy.201706008
文献标志码:
A
摘要:
目的 研究黄芩苷对高脂-脂肪肝模型大鼠肝脏炎症改善及炎性因子表达的影响,探讨其分子作用机制。
方法 将4周龄Wistar大鼠随机分为正常对照组(C组)、高脂模型组(M组)和黄芩苷治疗组(Ba组),每组12只。C组大鼠投喂普通颗粒饲料,M组和Ba组大鼠投喂高脂饲料建立高脂-脂肪肝大鼠模型。3周后,C组和M组大鼠给予生理盐水、Ba组大鼠给予黄芩苷30 mg/(kg?d)灌胃30 d。末次给药后处死大鼠,分离收集血清及肝脏组织。应用生化分析仪检测血清三酰甘油(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)水平及肝脏组织TG、TC含量,采用苏木精-伊红染色进行肝组织病理分析,采用酶联免疫吸附试验方法测定肝脏组织肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)蛋白表达,分别采用荧光定量PCR和免疫组化方法检测肝脏组织环氧化物酶2(COX-2)、诱导型一氧化氮合酶(iNOS)mRNA和蛋白表达水平。
结果 与C组相比,M组大鼠血清TG、TC、HDL-C、LDL-C水平升高(F=5.68~30.16,P<0.05),肝指数及肝组织中TG、TC水平升高(F=30.13~40.24,P<0.05),组织病理学检查示肝细胞变性坏死和肝组织炎症损伤加重,肝组织中炎性因子TNF-α和IL-6的表达升高(F=48.16、36.33,P<0.05),肝组织COX-2和iNOS的表达升高(F=33.16、38.84,P<0.05)。与M组相比,Ba组大鼠血清血脂水平、肝组织中脂质含量和肝指数均明显降低(F=5.68~40.24,P<0.05),肝细胞脂肪变性和炎症损伤减轻,肝组织中TNF-α、IL-6以及COX-2、iNOS的表达明显降低(F=33.16~48.16,P<0.05)。
结论 黄芩苷对高脂-脂肪肝大鼠肝细胞变性和肝组织炎症损伤有较好的治疗作用,其机制可能与抑制炎症因子TNF-α和IL-6及炎症调控因子COX-2和iNOS的表达相关。
Abstract:
Objective  To investigate the effect of baicalin on the improvement of liver inflammation and the expression of inflammatory factors in a rat model of hyperlipidemia-fatty liver disease, as well as its molecular mechanism.
Methods  Wistar rats aged 4 weeks were randomly divided into normal control group (group C), hyperlipidemia group (group M), and baicalin treatment group (group Ba), with 12 rats in each group. The rats in group C were given normal pellet diet, and those in group M and group Ba were given high-fat diet to establish a rat model of hyperlipidemia-fatty liver disease. Three weeks later, the rats in group C and group M were given normal saline by gavage, and those in group Ba were given baicalin 30 mg/(kg?d) by gavage; the course of treatment was 30 days for all groups. The rats were sacrificed after the last administration, and the serum and liver tissue were collected. A biochemical analyzer was used to measure the serum levels of triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C), as well as the levels of TG and TC in liver tissue. Hematoxylin and eosin staining was performed to evaluate liver pathology. ELISA was used to measure the protein expression of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in liver tissue, and quantitative real-time PCR and immunohistochemistry were used to measure the mRNA and protein expression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) in liver tissue.
Results  Compared with group C, group M had significant increases in the serum levels of TC, TG, HDL-C, and LDL-C (F=5.68-30.16,P<0.05), as well as liver index and the levels of TC and TG in liver tissue (F=30.13-40.24,P<0.05), aggravated hepatocyte degeneration and necrosis and liver inflammation, and significantly higher expression of TNF-α, IL-6, COX-2, and iNOS in liver tissue (F=33.16-48.16,P<0.05). Compared with group M, group Ba had significant reductions in the serum levels of blood lipids, the levels of lipids in liver tissue, and liver index (F=5.68-40.24,P<0.05), improvements in hepatocyte fatty degeneration and liver inflammation, and significant reductions in the expression of TNF-α, IL-6, COX-2, and iNOS in liver tissue (F=33.16-48.16,P<0.05).
Conclusion  Baicalin exhibits a good therapeutic effect on hepatocyte degeneration and liver inflammation in a rat model of hy perlipidemia-fatty liver disease, possibly by inhibiting inflammatory factors TNF-α and IL-6 and inflammatory regulation factors COX-2 and iNOS.
更新日期/Last Update: 2018-03-24