[1]苗玉,贺荟静,李斐斐,等. miR-205-5p对鼻咽癌细胞系增殖、迁移和侵袭的影响及其机制[J].青岛大学学报(医学版),2018,54(03 ):257-262.[doi:10.11712/jms201803002]
 MIAO Yu,HE Huijing,LI Feifei,et al. EFFECT OF MIR-205-5P ON PROLIFERATION, MIGRATION, AND INVASION OF NASOPHARYNGEAL CARCINOMA CELL LINE AND RELATED MECHANISM[J].JOURNAL OF QINGDAO UNIVERSITY (MEDICAL SCIENCES),2018,54(03 ):257-262.[doi:10.11712/jms201803002]
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 miR-205-5p对鼻咽癌细胞系增殖、迁移和侵袭的影响及其机制()
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《青岛大学学报(医学版)》[ISSN:2096-5532/CN:37-1217/R]

卷:
第54卷
期数:
2018年03 期
页码:
257-262
栏目:
出版日期:
2018-05-29

文章信息/Info

Title:
 EFFECT OF MIR-205-5P ON PROLIFERATION, MIGRATION, AND INVASION OF NASOPHARYNGEAL CARCINOMA CELL LINE AND RELATED MECHANISM
作者:
 苗玉贺荟静李斐斐王云
 青岛大学基础医学院病原生物学系,山东 青岛 266021
Author(s):
 MIAO Yu HE Huijing LI Feifei WANG Yun
 Department of Pathogenic Biology, School of Basic Medicine, Qingdao University, Qingdao 266021, China
关键词:
 微RNAs鼻咽肿瘤细胞增殖细胞运动肿瘤侵润
Keywords:
 microRNAs nasopharyngeal neoplasms cell proliferation cell movement neoplasm invasiveness
分类号:
R739.63
DOI:
10.11712/jms201803002
文献标志码:
A
摘要:
 目的 观察微小RNA miR-205-5p对鼻咽癌(NPC)细胞系HONE1增殖、迁移和侵袭的影响,探讨其作用机制。
方法 将人工合成的miR-205-5p模拟物、抑制物及相应阴性对照转染至HONE1细胞中,用CCK-8法检测细胞增殖,细胞划痕实验及Transwell小室检测细胞迁移和侵袭,Western blot检测miR-205-5p潜在靶基因PTEN、Smad4以及PTEN下游基因蛋白激酶B(AKT)的表达水平。
结果 与对照相比,miR-205-5p模拟物转染细胞增殖、迁移和侵袭能力增强,PTEN和Smad4表达下降,磷酸化AKT(p-AKT)表达升高;而miR-205-5p抑制物转染细胞增殖、迁移和侵袭能力减弱,PTEN和Smad4表达升高,p-AKT表达下降,差异均有统计学意义(t=2.970~8.011,P<0.05)。
结论 miR-205-5p可促进NPC细胞增殖、迁移和侵袭,其机制可能与靶向调控抑癌基因PTEN和Smad4表达有关。
Abstract:
 Objective To investigate the effect of miR-205-5p on the proliferation, migration, and invasion of nasopharyngeal carcinoma (NPC) cell line HONE1 and the related mechanism.
Methods Synthesized miR-205-5p mimics and inhibitor and their negative controls were separately transfected into HONE1 cells. CCK-8 assay was used to assess cell proliferation; wound-healing assay and the Transwell test were used to evaluate cell migration and invasion; Western blot was used to measure the expression of potential target genes of miR-205-5p (PTEN and Smad4) and the downstream gene of PTEN (protein kinase B (AKT)).
Results Compared with the control group, the miR-205-5p mimic transfection group had significant increases in the abilities of cell proliferation, migration, and invasion, significant reductions in the expression of PTEN and Smad4, and a significant increase in the expression of phosphorylated AKT (p-AKT), while the miR-205-5p inhibitor transfection group had significant reductions in the abilities of cell proliferation, migration, and invasion, significant increases in the expression of PTEN and Smad4, and a significant reduction in the expression of p-AKT (t=2.970-8.011,P<0.05).
Conclusion MiR-205-5p may promote the proliferation, migration, and invasion of NPC cell line HONE1, possibly by targeted regulation of the expression of tumor suppressor genes PTEN and Smad4.
更新日期/Last Update: 2018-06-05