[1]仇晓晨,蒋梦会,朴金梅,等. 适量铜对BALB/c小鼠结直肠癌肝转移的影响[J].青岛大学学报(医学版),2018,54(03 ):263-267,272.[doi:10.11712/jms201803003]
 QIU Xiaochen,JIANG Menghui,PIAO Jinmei,et al. EFFECT OF AN APPROPRIATE AMOUNT OF COPPER ON COLORECTAL CANCER LIVER METASTASIS IN BALB/C MICE[J].JOURNAL OF QINGDAO UNIVERSITY (MEDICAL SCIENCES),2018,54(03 ):263-267,272.[doi:10.11712/jms201803003]
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 适量铜对BALB/c小鼠结直肠癌肝转移的影响()
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《青岛大学学报(医学版)》[ISSN:2096-5532/CN:37-1217/R]

卷:
第54卷
期数:
2018年03 期
页码:
263-267,272
栏目:
出版日期:
2018-05-29

文章信息/Info

Title:
 EFFECT OF AN APPROPRIATE AMOUNT OF COPPER ON COLORECTAL CANCER LIVER METASTASIS IN BALB/C MICE
作者:
 仇晓晨蒋梦会朴金梅宋扬杨垚崔莲花
 青岛大学医学院营养研究所,山东 青岛 266021
Author(s):
 QIU Xiaochen JIANG Menghui PIAO Jinmei SONG Yang YANG Yao CUI Lianhua
 Department of Public Health, Medical College, Qingdao University, Qingdao 266021, China
关键词:
 结直肠肿瘤肿瘤转移基质金属蛋白酶7血管内皮生长因子类
Keywords:
 copper colorectal neoplasms neoplasm metastasis liver matrix metalloproteinase 7 vascular endothelial growth factors
分类号:
R348.1;R73-37
DOI:
10.11712/jms201803003
文献标志码:
A
摘要:
 目的 通过建立BALB/c小鼠结直肠癌肝转移模型探讨铜对肝转移及转移相关蛋白的影响。
方法 采用脾脏注射合并去脾法建立结直肠癌肝转移模型。将48只雄性BALB/c小鼠按照体质量随机分为4组,每组12只,分别是对照组、铜组(0.3 mg/kg)、四硫钼酸铵盐(TM)组(1.0 mg)、铜+TM组(0.3 mg/kg铜+1.0 mg TM)。采用灌胃方式提前干预1周,术后第2天开始,给予同样的灌胃方式,同时TM组和铜+TM组给予1.0 mg 的TM,持续4周左右处死小鼠,观察瘤结节数和肝转移率。采用免疫组化法检测基质金属蛋白酶7(MMP7)和血管内皮生长因子(VEGF)与转移有关因子的表达。
结果 各组小鼠体质量增长趋势大致相同,实验期间体质量变化无明显差异(F=0.749,P>0.05)。铜组较对照组和铜+TM组的瘤结节数显著增多(t=5.93、8.64,P<0.05),对照组与TM组比较差异有统计学意义(t=2.38,P<0.05)。铜组肝转移率较其他3组均有所升高。铜组与对照组及铜+TM组比较,MMP7和VEGF蛋白的表达有所升高,差异具有统计学意义(F=7.064、31.595,P<0.05)。
结论  适量铜可以促进结直肠癌肝转移肿瘤的发生,引起转移相关蛋白表达变化,从而促进结直肠癌的发展进程。
Abstract:
 Objective To investigate the effect of copper on liver metastasis and metastasis-associated proteins by establishing a BALB/c mouse model of colorectal cancer liver metastasis.
Methods Spleen injection combined with splenectomy was used to establish a model of colorectal cancer liver metastasis. A total of 48 male BALB/c mice were randomly divided into control group, copper group (0.3 mg/kg), tetrathiomolybdate (TM) group (1.0 mg), and copper+TM group (0.3 mg/kg copper+1.0 mg TM) according to body weight, with 12 mice in each group. Copper was given by gavage for 1 week in advance. On day 2 after surgery, the mice were treated with the same drug by gavage, and those in the TM group and the copper+TM group were treated with 1.0 mg TM. The course of treatment was 4 weeks, and then the mice were sacrificed to observe the number of tumor nodules and liver metastasis rate. Immunohistochemistry was used to measure the expression of matrix metalloproteinase 7 (MMP7), vascular endothelial growth factor (VEGF), and metastasis-associated factors.
Results All groups had a similar trend in the increase in body weight, and there was no significant change in body weight during the experiment (F=0.749,P>0.05). Compared with the control group and the copper+TM group, the copper group had a significant increase in the number of tumor nodules (t=5.93,8.64;P<0.05), and there was a significant difference in this number between the control group and the TM group (t=2.38,P<0.05). The copper group had a higher liver metastasis rate than the other three groups. Compared with the control group and the copper+TM group, the copper group had significant increases in the protein expression of MMP7 and VEGF (F=7.064 and 31.595,P<0.05).
Conclusion An appropriate amount of copper can promote colorectal cancer liver metastasis, cause the changes in the expression of metastasis-associated proteins, and thus promote the progression of colorectal cancer.
更新日期/Last Update: 2018-06-05