[1]付兴芹,张惠,张青青,等. 牛蒡子苷元对ConA诱导小鼠急性肝炎的保护作用[J].青岛大学学报(医学版),2018,54(03 ):268-272.[doi:10.11712/jms201803004]
 FU Xingqin,ZHANG Hui,ZHANG Qingqing,et al. PROTECTIVE EFFECT OF ARCTIGENIN AGAINST CONCANAVALIN A-INDUCED ACUTE HEPATITIS IN MICE[J].JOURNAL OF QINGDAO UNIVERSITY (MEDICAL SCIENCES),2018,54(03 ):268-272.[doi:10.11712/jms201803004]
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 牛蒡子苷元对ConA诱导小鼠急性肝炎的保护作用()
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《青岛大学学报(医学版)》[ISSN:2096-5532/CN:37-1217/R]

卷:
第54卷
期数:
2018年03 期
页码:
268-272
栏目:
出版日期:
2018-05-29

文章信息/Info

Title:
 PROTECTIVE EFFECT OF ARCTIGENIN AGAINST CONCANAVALIN A-INDUCED ACUTE HEPATITIS IN MICE
作者:
 付兴芹12张惠2张青青2姚潇颖2朱玉贞1司传平2
 1 青岛大学基础医学院免疫学系,山东 青岛 266071; 2 济宁医学院免疫学与分子医学研究所
Author(s):
 FU Xingqin ZHANG Hui ZHANG Qingqing YAO Xiaoying ZHU Yuzhen SI Chuanping
 Department of Immunology, Medical College of Qingdao University, Qingdao 266071, China
关键词:
 牛蒡子苷元肝炎刀豆蛋白A肿瘤坏死因子α干扰素γ
Keywords:
 arctigenin hepatitis concanavalin A tumor necrosis factor-alpha interferon-gamma
分类号:
R575.1
DOI:
10.11712/jms201803004
文献标志码:
A
摘要:
 目的 研究牛蒡子苷元(ATG)对刀豆蛋白A(ConA)诱导的小鼠急性肝炎的保护作用。
方法 将C57BL/6小鼠随机分成DMSO组、ConA/DMSO组、ConA/ATG组,每组10只小鼠。DMSO组和ConA/DMSO组小鼠腹腔注射DMSO,ConA/ATG组小鼠腹腔注射等体积ATG(30 mg/kg),5 h后ConA/DMSO组和ConA/ATG组尾静脉注射ConA(20 mg/kg)。12 h后处死小鼠,取血清和肝脏组织,用生化分析仪测血清丙氨酸氨基转移酶(ALT)和天门冬氨酸氨基转移酶(AST)水平,苏木精-伊红(HE)染色检测肝组织病理损伤,TUNEL染色检测肝细胞凋亡,ELISA方法检测血清肿瘤坏死因子α(TNF-α)和干扰素-γ(IFN-γ)水平,q-PCR方法检测肝组织中TNF-α和IFN-γ mRNA表达水平,流式细胞术检测骨髓来源抑制细胞(MDSCs),生化法检测肝脏匀浆丙二醛(MDA)和髓过氧化物酶(MPO)的表达水平。
结果 与DMSO组相比,ConA/DMSO组血清ALT和AST水平明显升高(F=125.80、94.74,P<0.05);肝脏组织损伤明显加重;肝细胞凋亡增加;血清和肝组织中TNF-α和IFN-γ水平升高(F=17.52、68.81,P<0.05);肝脏MDSCs募集活化降低;同时MDA和MPO活性升高(F=13.38、14.78,P<0.05)。与ConA/DMSO组比较,ConA/ATG组血清ALT和AST水平明显降低(F=125.80、94.74,P<0.05);肝脏组织损伤明显减轻;肝细胞凋亡减少;血清中TNF-α和IFN-γ的水平降低(F=17.52、68.81,P<0.05);肝组织中TNF-α和IFN-γ mRNA的表达水平降低(F=31.55、14.64,P<0.05),MDSCs募集活化明显增强;MDA和MPO活性降低(F=13.38、14.78,P<0.05)。
结论 ATG对ConA诱导的小鼠急性肝炎具有明显的保护作用。
Abstract:
 Objective To investigate the protective effect of arctigenin (ATG) against concanavalin A (ConA)-induced acute hepatitis in mice.
Methods C57BL/6 mice were randomly divided into DMSO group, ConA/DMSO group, and ConA/ATG group, with 10 mice in each group. The mice in the DMSO group and the ConA/DMSO group were given intraperitoneal injection of DMSO, and those in the ConA/ATG group were given intraperitoneal injection of the same volume of ATG (30 mg/kg); 5 h later, the mice in the ConA/DMSO group and the ConA/ATG group were given tail vein injection of ConA (20 mg/kg). The mice were sacrificed 12 h later, and serum and liver tissue samples were collected. An automatic biochemical analyzer was used to measure the serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST); HE staining was used to observe liver pathological injury; TUNEL staining was used to measure cell apoptosis; ELISA was used to measure the serum levels of tumor necrosis factor-α (TNF-α) and interferon gamma (IFN-γ); q-PCR was used to measure the mRNA expression of TNF-α and IFN-γ in liver tissue; flow cytometry was used to measure myeloid-derived suppressor cells (MDSCs); and the biochemical method was used to measure the expression of malondialdehyde (MDA) and myeloperoxidase (MPO) in liver homogenate.
Results Compared with the DMSO group, the ConA/DMSO group had significant increases in the serum levels of ALT and AST (F=125.80 and 94.74,P<0.05), significantly greater liver injury, significant increases in hepatocyte apoptosis and the levels of TNF-α and IFN-γ in serum and liver tissue (F=17.52 and 68.81,P<0.05), a significant reduction in recruitment of MDSCs in the liver, and significant increases in the activities of MDA and MPO (F=13.38 and 14.78,P<0.05). Compared with the ConA/
DMSO group, the ConA/ATG group had significant reductions in the serum levels of ALT and AST (F=125.80 and 94.74,P<0.05), significant alleviation of liver injury, a significant reduction in hepatocyte apoptosis, significant reductions in the serum level of TNF-α and IFN-γ (F=17.52 and 68.81,P<0.05) and the mRNA expression of TNF-α and IFN-γ in liver tissue (F=31.55 and 14.64,P<0.05), a significant increase in recruitment of MDSCs in the liver, and significant reductions in the activities of MDA and MPO (F=13.38 and 14.78,P<0.05).
Conclusion ATG has a marked protective effect against ConA-induced acute hepatitis in mice.
更新日期/Last Update: 2018-06-05