[1]丁蕾,钟亮,王素萍,等. NRG-1β对大鼠脑缺血再灌注损伤后造血祖细胞激酶1表达影响[J].青岛大学学报(医学版),2018,54(03 ):273-276.[doi:10.11712/jms201803005]
 DING Lei,ZHONG Liang,WANG Suping,et al. EFFECT OF NRG-1β ON EXPRESSION OF HPK1 AFTER CEREBRAL ISCHEMIA-REPERFUSION INJURY IN RATS[J].JOURNAL OF QINGDAO UNIVERSITY (MEDICAL SCIENCES),2018,54(03 ):273-276.[doi:10.11712/jms201803005]
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 NRG-1β对大鼠脑缺血再灌注损伤后造血祖细胞激酶1表达影响()
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《青岛大学学报(医学版)》[ISSN:2096-5532/CN:37-1217/R]

卷:
第54卷
期数:
2018年03 期
页码:
273-276
栏目:
出版日期:
2018-05-29

文章信息/Info

Title:
 EFFECT OF NRG-1β ON EXPRESSION OF HPK1 AFTER CEREBRAL ISCHEMIA-REPERFUSION INJURY IN RATS
作者:
 丁蕾12钟亮3王素萍3季亚清1于竹芹4郭云良1
 1 青岛大学医学部中西医结合中心,山东 青岛 266021; 2 青岛市第三人民医院皮肤科;3 青岛市第三人民医院康复科; 4 青岛大学医学院松山医院
Author(s):
 DING Lei ZHONG Liang WANG Suping JI Yaqing YU Zhuqin GUO Yunliang
 Institute of Integrative Medicine Center, Qingdao University Medical College, Qingdao 266021, China
关键词:
 神经调节蛋白1脑缺血再灌注损伤造血祖细胞激酶1
Keywords:
 neuregulin-1 brain ischemia reperfusion injury hematopoietic progenitor kinase-1
分类号:
R977.6;R364.1
DOI:
10.11712/jms201803005
文献标志码:
A
摘要:
 目的 探讨神经调节素1β(NRG-1β)对大鼠脑缺血再灌注损伤后造血祖细胞激酶1(HPK1)表达的影响和意义。
方法 将Wistar大鼠随机分为假手术组、模型组和NRG-1β治疗组。采用改良Longa法制备大鼠脑缺血2 h再灌注24 h模型。采用改良神经功能缺损评分(mNSS评分)评价各组大鼠神经行为功能,苏木精-伊红染色检测神经细胞病理形态,TUNEL染色检测神经细胞凋亡,免疫组化染色和Westem blot检测HPK1表达水平。
结果 与假手术组相比,模型组大鼠mNSS评分升高,神经细胞形态结构异常,凋亡细胞增多,HPK1蛋白表达增强;与模型组相比,NRG-1β治疗组大鼠mNSS评分降低,神经行为功能改善,神经细胞的形态结构异常减轻,凋亡细胞数降低,HPK1蛋白表达降低,差异均有统计学意义(F=15.1~22.4,P<0.05)。
结论 NRG-1β可通过抑制脑缺血再灌注后HPK1的表达抗细胞凋亡,发挥神经保护作用。
Abstract:
 Objective To investigate the effect of neuregulin-1β (NRG-1β) on the expression of hematopoietic progenitor kinase-1 (HPK1) after cerebral ischemia-reperfusion injury in rats and its significance.
Methods Wistar rats were randomly divided into sham-operation group, model group, and NRG-1β treatment group. The modified Longa method was adopted to prepare the rat model with 2 h of cerebral ischemia followed by 24 h of reperfusion. The neurobehavioral function of rats was evaluated by modified Neurological Severity Score (mNSS). Hematoxylin and eosin staining was used to observe the pathological morphology of neural cells. Apoptosis of neural cells was determined by TUNEL staining. The expression level of HPK1 was determined by immunohistochemical staining and Western blot.
Results Compared with the sham-operation group, the model group showed increased mNSS score, abnormal morphological structure of neural cells, an increased number of apoptotic cells, and higher protein expression of HPK1. Compared with the model group, the NRG-1β treatment group showed decreased mNSS score, improved neurobehavioral function, reduced morphological and structural abnormalities of neural cells, a decreased number of apoptotic cells, and reduced protein expression of HPK1 (F=15.1-22.4,P<0.05).
Conclusion NRG-1β can inhibit cell apoptosis and play a role in neuroprotection through inhibiting the expression of HPK1 after cerebral ischemia-reperfusion injury.
更新日期/Last Update: 2018-06-05