[1]许春爽,周易,姜宙,等. 赤霉素对精子运动力和细胞凋亡的影响及其机制[J].青岛大学学报(医学版),2018,54(03 ):282-286.[doi:10.11712/jms201803007]
 XU Chunshuang,ZHOU Yi,JIANG Zhou,et al. EFFECTS OF GIBBERELLIN ON HUMAN SPERM MOTILITY AND APOPTOSIS IN VITRO AND ITS MECHANISM[J].JOURNAL OF QINGDAO UNIVERSITY (MEDICAL SCIENCES),2018,54(03 ):282-286.[doi:10.11712/jms201803007]
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 赤霉素对精子运动力和细胞凋亡的影响及其机制()
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《青岛大学学报(医学版)》[ISSN:2096-5532/CN:37-1217/R]

卷:
第54卷
期数:
2018年03 期
页码:
282-286
栏目:
出版日期:
2018-05-29

文章信息/Info

Title:
 EFFECTS OF GIBBERELLIN ON HUMAN SPERM MOTILITY AND APOPTOSIS IN VITRO AND ITS MECHANISM
作者:
 许春爽1周易1姜宙1臧丽丽1王丽娥1邹淑花12
 1 青岛大学附属青岛市妇女儿童医院生殖中心,山东 青岛 266034; 2 上海国际和平妇幼保健院生殖中心
Author(s):
 XU Chunshuang ZHOU Yi JIANG Zhou ZANG Lili WANG Li′e ZOU Shuhua
 Reproductive Center of Qingdao Women and Children Hospital Affiliated to Qingdao University, Qingdao 266034, China
关键词:
 赤霉素类精子能动性氧化性应激细胞凋亡腺苷三磷酸酶类
Keywords:
 gibberellins sperm motility oxidative stress apoptosis adenosine triphosphatases
分类号:
R978.15;R321.1
DOI:
10.11712/jms201803007
文献标志码:
A
摘要:
 目的 探讨赤霉素对精子运动力和细胞凋亡的影响及其机制。
方法 采用Percoll梯度离心法提取有正常生理功能的人精子作为正常精子模型,提取的优质精子培养于含不同浓度赤霉素的G-IVF PLUS培养液中,采用精子质量分析仪检测精子运动力;流式细胞分析仪检测精子细胞活性氧(ROS)水平及凋亡情况;免疫荧光技术检测氧化应激、凋亡相关蛋白表达量;ATP酶检测试剂盒检测Na+-K+-ATP酶和Ca2+-ATP酶活性。
结果 精子运动力随赤霉素浓度的增加、时间的延长逐渐降低;处理组精子细胞ROS水平明显高于对照组(F=21.40,P<0.05),过氧化物歧化酶(SOD)蛋白水平较对照组明显降低(F=28.13,P<0.05);凋亡蛋白P53、BAX表达水平较对照组明显升高(F=104.54、59.29,P<0.05);Na+-K+-ATP酶和Ca2+-ATP酶活性较对照组明显降低(F=24.48、18.81,P<0.05)。
结论 赤霉素可通过氧化应激反应降低精子运动力,促进精子细胞凋亡。
Abstract:
 Objective To investigate the effects of gibberellin on human sperm motility and apoptosis in vitro and its mechanism.
Methods The human sperms with normal physiological function were selected as normal control group using a discontinuous Percoll gradient centrifugation (45%-90%). The high-quality sperms (treatment group) were exposed to G-IVF PLUS medium with different concentrations of gibberellin in vitro. The sperm motility was measured by a sperm quality analyzer at different time points. Flow cytometry was used to determine the level of reactive oxygen species (ROS) and apoptosis. Immunofluorescence was used to measure the expression of proteins related to oxidative stress and apoptosis. The activity of Na+-K+-ATP enzyme and Ca2+-ATP enzyme were determined by an ATP enzyme assay kit.
Results The sperm motility gradually decreased with time and the increase in gibberellin concentration. Compared with the control group, the treatment group had a significantly higher ROS level (F=21.40,P<0.05) and a significantly lower level of superoxide dismutase (F=28.13,P<0.05); moreover, the treatment group had significantly higher expression of P53 and BAX (apoptotic proteins) (F=104.54,59.29;P<0.05) and significantly lower activity of Na+-K+-ATP enzyme and Ca2+-ATP enzyme (F=24.48,18.81;P<0.05).
Conclusion Gibberellin can reduce sperm motility and promote sperm apoptosis via oxidative stress reaction.
更新日期/Last Update: 2018-06-05