[1]朱明宇,李雨珊,王志国,等. 机械张力加载时间对成纤维细胞机械传导效应影响[J].青岛大学学报(医学版),2018,54(03 ):313-316,320.[doi:10.11712/jms201803014]
 ZHU Mingyu,LI Yushan,WANG Zhiguo,et al. EFFECT OF TIME UNDER MECHANICAL TENSION ON MECHANOTRANSDUCTION OF FIBROBLASTS[J].JOURNAL OF QINGDAO UNIVERSITY (MEDICAL SCIENCES),2018,54(03 ):313-316,320.[doi:10.11712/jms201803014]
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 机械张力加载时间对成纤维细胞机械传导效应影响()
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《青岛大学学报(医学版)》[ISSN:2096-5532/CN:37-1217/R]

卷:
第54卷
期数:
2018年03 期
页码:
313-316,320
栏目:
出版日期:
2018-05-29

文章信息/Info

Title:
 EFFECT OF TIME UNDER MECHANICAL TENSION ON MECHANOTRANSDUCTION OF FIBROBLASTS
作者:
 朱明宇1李雨珊2王志国1匡瑞霞1
 1 青岛大学附属医院整形美容科,山东 青岛 266003; 2 安阳市人民医院整形美容外科
Author(s):
 ZHU Mingyu LI Yushan WANG Zhiguo KUANG Ruixia
 Department of Plastic Surgery, The Affiliated Hospital of Qingdao University, Qingdao 266003, China
关键词:
 成纤维细胞瘢痕皮肤机械感受器整合素类
Keywords:
 fibroblasts cicatrix skin mechanoreceptors integrins
分类号:
R329.24;R339.19
DOI:
10.11712/jms201803014
文献标志码:
A
摘要:
 目的 探讨加载机械张力参数为拉伸幅度的10%时,机械张力的加载时间对正常皮肤成纤维细胞(NSFB)机械传导效应的影响。
方法 体外培养人体NSFB和增生性瘢痕成纤维细胞(HSFB),NSFB和HSFB分别设实验组和对照组,实验组加载机械张力参数为拉伸幅度10%,对照组不加载机械张力。在加载周期性机械张力3、5、7 d时,用CCK-8检测各组细胞增殖能力,用Real-time PCR法检测各组细胞Integrin-β1、P130Cas基因表达水平,用Western-Blot方法检测细胞Integrin-β1、P130Cas蛋白表达水平。
结果 加载机械张力5 d时,NSFB实验组与HSFB对照组比较,细胞增殖、P130Cas和Integrin-β1的表达水平差异均无统计学意义(Z=-0.218,F=0.591、0.079,P>0.05);加载3、7 d时,NSFB实验组与HSFB对照组比较,细胞增殖、P130Cas、Integrin-β1表达差异均有统计学意义(Z=-5.294~-2.411,F=34.182~872.148,P<0.05)。
结论 拉伸幅度为10%的机械张力加载5 d时,可使NSFB表现出与HSFB相同的机械传导效应。
Abstract:
 Objective To investigate the effect of time under mechanical tension (TUMT) on the mechanotransduction of normal skin fibroblasts (NSFB) when mechanical tension was set to 10% stretch amplitude.
Methods Human NSFB and hypertrophic scar fibroblasts (HSFB) were cultured in vitro as test group and control group, respectively. The test group was loaded with mechanical tension of 10% stretch amplitude, while the control group was loaded with zero tension. When cyclic TUMT reached 3, 5, and 7 d, cell proliferation in each group was determined by CCK-8 assay, while the mRNA and protein expression of Integrin-β1 and P130Cas in each group were measured by real-time PCR and Western blot, respectively.
Results When TUMT was 5 d, there were no significant differences between the test group and the control group in cell proliferation and the expression of Integrin-β1 and P130Cas (Z=-0.218,F=0.591 and 0.079,P>0.05); but when TUMT was 3 or 7 d, there were significant differences between the test group and the control group in cell proliferation and the expression of Integrin-β1 and P130Cas (Z=-5.294 to -2.411,F=34.182-872.148,P<0.05).
Conclusion When TUMT of 10% stretch amplitude is 5 d, NSFB may exhibit the same mechanotransduction as HSFB.
更新日期/Last Update: 2018-06-05