[1]张海洋,钱国武,王鑫,等.RNAi TRAF4表达对直肠癌细胞凋亡及Wnt信号通路相关蛋白表达影响[J].青岛大学学报(医学版),2018,54(04):415-418,422.[doi:10.11712/jms201804009]
 ZHANG Haiyang,QIAN Guowu,WANG Xin,et al.EFFECT OF RNA INTERFERENCE OF TRAF4 EXPRESSION ON APOPTOSIS AND WNT SIGNALING PATHWAY IN RECTAL CANCER CELLS[J].JOURNAL OF QINGDAO UNIVERSITY (MEDICAL SCIENCES),2018,54(04):415-418,422.[doi:10.11712/jms201804009]
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RNAi TRAF4表达对直肠癌细胞凋亡及Wnt信号通路相关蛋白表达影响()
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《青岛大学学报(医学版)》[ISSN:2096-5532/CN:37-1217/R]

卷:
第54卷
期数:
2018年04期
页码:
415-418,422
栏目:
出版日期:
2018-07-05

文章信息/Info

Title:
EFFECT OF RNA INTERFERENCE OF TRAF4 EXPRESSION ON APOPTOSIS AND WNT SIGNALING PATHWAY IN RECTAL CANCER CELLS
文章编号:
2096-5532(2018)04-0415-05
作者:
张海洋1钱国武2王鑫3姚坤厚4
(1 南阳市中心医院普外科,河南 南阳 473000; 2 南阳市中心医院实验室; 3 郑州大学第一附属医院放疗一科; 4 河南大学淮河医院普外科)
Author(s):
ZHANG Haiyang QIAN Guowu WANG Xin YAO Kunhou
(Department of General Surgery, Nanyang Central Hospital, Nanyang 473000, China)
关键词:
直肠肿瘤TNF受体相关因子4RNA干扰细胞凋亡Wnt信号通路
Keywords:
rectal neoplasms TNF receptor-associated factor 4 RNA interference apoptosis Wnt signaling pathway
分类号:
R735.37;R394.114
DOI:
10.11712/jms201804009
文献标志码:
A
摘要:
目的 探讨RNA干扰(RNAi)肿瘤坏死因子受体相关因子4(TRAF4)表达对直肠癌细胞凋亡及Wnt信号通路相关蛋白表达的影响。
方法 将TRAF4的siRNA转染人直肠癌SW1463细胞(TRAF4-siRNA组),并设置阴性对照组(NC组)及空白组。收集转染48 h的3组细胞,通过Western blotting方法检测3组细胞中TRAF4的蛋白表达,流式细胞仪检测各组细胞的凋亡率及活性氧簇(ROS)含量,Western blotting检测凋亡蛋白survivin、Bcl-2相关X蛋白(Bax)和Wnt信号通路相关蛋白β-连环蛋白(β-catenin)和细胞周期素D1(cyclin D1)的蛋白表达。
结果 TRAF4-siRNA组TRAF4的蛋白表达显著低于空白组(t=7.926,P<0.05),NC组TRAF4蛋白表达与空白组差异无统计学意义(t=0.634,P>0.05);与空白组比较,TRAF4-siRNA组细胞凋亡率显著升高(t=14.992,P<0.05),ROS含量显著上升(t=5.717,P<0.05),survivin、β-catenin和cyclin D1的蛋白表达显著降低(t=5.437~9.344,P<0.05),Bax的蛋白表达显著升高(t=7.399,P<0.05)。
结论 抑制直肠癌细胞中TRAF4基因表达可诱导细胞凋亡及下调Wnt信号通路。
Abstract:
Objective To investigate the effect of RNA interference of tumor necrosis factor receptor-associated factor 4 (TRAF4) expression on apoptosis and Wnt signaling pathway in rectal cancer cells.
Methods The human colorectal cancer cell line SW1463 transfected with small interfering RNA (siRNA) of TRAF4 was set as TRAF4-siRNA group. Meanwhile, negative control group (NC group) and blank group were established. Cells were collected from the three groups after 48 h transfection. For all the three groups, Western blotting was used to measure the protein expression of TRAF4, survivin, Bcl-2 related X protein (Bax), and Wnt signaling pathway-associated proteins (β-catenin and cyclin D1); the cell apoptosis rate and reactive oxygen species (ROS) content were determined by flow cytometry.
Results Compared with the blank group, the TRAF4-siRNA group had significantly lower expression of TRAF4 protein (t=7.926,P<0.05), but there was no significant difference in the expression of TRAF4 protein between the blank group and the NC group (t=0.634,P>0.05). Compared with the blank group, the TRAF4-siRNA group had significantly increased apoptosis rate (t=14.992,P<0.05) and ROS content (t=5.717,P<0.05), significantly reduced expression of survivin, β-catenin, and cyclin D1 proteins (t=5.437-9.344,P<0.05), and significantly elevated expression of Bax (t=7.399,P<0.05).
Conclusion Inhibition of TRAF4 gene expression in rectal cancer cells can induce apoptosis and downregulate the Wnt signaling pathway.
更新日期/Last Update: 2018-07-08